Amidst the havoc of the corona virus, there is only one word on the tongue of the whole world… vaccine. Many countries have given this ‘safety shield’ to their citizens, while many countries are still struggling with the lack of vaccine. But do you know how the corona vaccine is made? The Pfizer-BioNtech vaccine takes 60 days to prepare. During this, he has to go through about 18 difficult stages.
1/10
First the scientist extracts a vial of DNA from the master cell bank. Every COVID-19 vaccine contains this DNA. These vials are kept at a temperature of less than 150 ° C and contain a small ring of DNA called plasmids. Each plasmid has a coronavirus gene. There are instructions in this way for a human cell to make a coronavirus protein. After this, the plasmid is added to the scientist by melting the plasmid and modifying the E. coli bacteria. 5 crore vaccines can be prepared from this single vial. AFP
2/10
The modified bacteria are placed in a flask and rotated and then placed in a warm environment to allow the bacteria to multiply. After allowing the bacteria to grow overnight, they are put in a large fermenter. It contains up to 300 liters of nutritious fluid. It is left as is for four days. These bacteria multiply every 20 minutes. In this process, billions of plasmid DNA are formed. AFP
3/10
According to a New York Times news, after the completion of the formulation, scientists separate the bacteria by mixing the chemical. The plasmid is then separated from the cells. The mixture is then filtered and the bacteria removed. After this only plasmids are left. Then their purity is checked and comparing it with the previous sample, it is seen that there is no change in its gene sequence. After checking the purity of the plasmid, a protein named Enzymes is added to it. It cleaves the coronavirus gene by cutting the plasmid. This process takes two days. AFP
4/10
The remaining bacteria or plasmids are thoroughly filtered, giving one liter bottle of purified DNA. The sequence of the DNA is rechecked and kept as a template for the next process. 1.5 lakh vaccines are prepared from one bottle of DNA. Each bottle of DNA is freeze and sealed. After that it is locked in the box with a small monitor. This monitor records its temperature on the way. 48 bags are kept in a container and covered with lots of dry ice so that its temperature remains minus 20 degrees. AFP
5/10
These canteeners are locked so that there is no tampering and then it is sent to Andover, Mass. Here, DNA is processed into RNA or mRNA. This is the main component of Pfizer’s vaccine. Other bottles are shipped to the Mainz plant in Germany. From here the vaccine is made for the market of Europe and other countries. The prepared vaccine works by sending mRNA to the human cell. This mixture is poured into a large tank and the filtrate removes excess DNA and other impurities. A dose of 75 lakh vaccines can be made from one batch. AFP
6/10
The accuracy of scientific repeated filtered mRNA is checked and confirmed that the gene sequence is correct. After this, again, the bag is stored at minus 20 degrees and it is sent to Kalamazoo, Mich. Here the vaccine is finalized and filled in vials. From here the sample is sent to Chesterfield so that it can be examined. The bag is kept safe in Kalamaju so that the vaccine can be made if needed. AFP
7/10
In a separate process, scientists prepare an oily lipid. This mRNA is kept safe and it helps to reach human cells. Lipids are mixed with ethanol. However, it is removed after the vaccine is ready. Ethanol and other items are removed from the vaccine and ultimately sterilized. AFP
8/10
After this the vials are prepared. Thousands of vials are washed and disinfected. 13 high-speed cameras take at least 100 pictures of a bottle. If there is any breakage or other deficiency in any vial, then it is removed. In a separate machine, each vial is vacuumed to find out if it is leaking. AFP
9/10
Each vial is filled with 0.45 millimeters of medicine. It can be vaccinated to six people. In one minute 575 vials are filled and sealed with a blue lid. The vaccine is freezed and heated immediately because the mRNA may be spoiled by being cold for too long. But all this work has to be completed within 46 hours. AFP
10/10
The filled vials are checked again. They are then labeled and packed in a plastic pizza box. This box contains 195 vials. A bundle of 5 such boxes is made and packed in 350 industrial freezers. 300 trays are kept in a freezer. It takes two days for the vaccine to reach minus 70 degrees. After freezing, the vaccine is left for testing for four weeks. The samples are then sent to Andover. After testing, it is sent to all countries. But order of at least 195 vials is necessary. AFP
.